Abstract: The organizational atmosphere perception and satisfaction of 236 staff were measured with psychological scale. To overcome shortcomings of K-means, quantum genetic algorithm was used to cluster the data of organizational atmosphere. Each dimensional data of cluster center was coded with 10 quantum bits. The chromosomes were iteratively evaluated with rotation gate. Three categories of staff were obtained. There were significant differences of organizational atmosphere and satisfaction scores among the categories. It means that the cluster analysis with quantum genetic algorithm is feasible. The result is valuable to improve the management level for the enterprise.